Health Benefits of Black Seed

Black seed has a wide range of health benefits and some of them are listed in Fig. 1.

black seed properties

Fig. 1. Health Benefits of Black Seed

Antiasthamatic Potential of Black Seed (Nigella sativa)

In a recent study, the immunomodulatory activity of black seed extracts (sample 1-4) were assessed by the release of mediators [Interleukin-2 (IL-2), Interleukin-6 (IL-6), Prostaglandin-E2 (PGE2)] in primary human T-lymphocytes, monocytes, and A549 human lung epithelial cells (Koshak et al., 2018). The details of the samples are given in Table 1.

Table 1. The Details of Samples Black Seed Extracts and Their Thymoquinone Concentration
Sl. NoSample ID%w/w Thymoquinone
1Ethanol Extract0.4
2Water Extract<0.005
3Nigellin® AMBER2.4
4Cold Pressed Oil0.7
Immunomodulatory activity of Black Seed Extracts
nigellin

Fig. 2A.

nigellin

Fig. 2B.

nigellin

Fig. 2C.

Fig. 2. Effects of Black Seed Extracts on IL-2 (Fig. 2A), IL-6 (Fig. 2B) and PGE2 (Fig. 2C) production by SEB-induced T-lympocyte cells
(n=3), (*p<0.05, **p<0.01, ***p<0.001), (adapted from Koshak et al., 2018).


nigellin

Fig. 3A.

nigellin

Fig. 3B.

Fig. 3. Effects of Black Seed Extracts on IL-6 (Fig. 3A) and PGE2 (Fig. 3B) release by LPS-induced Monocyte
(n=3), (*p<0.05, **p<0.01, ***p<0.001), (adapted from Koshak et al., 2018)


nigellin

Fig. 4. Effects of Black Seed Extracts on PGE2 production by A549 cell
(n=3), (*p<0.05, **p<0.01, ***p<0.001), (adapted from Koshak et al., 2018).

Nigellin® AMBER and the cold pressed oil which are characterized by the highest level of Thymoquinone, showed the most remarkable effect on suppressing inflammatory mediators IL-2, IL-6, and PGE2 release from human T-lymphocytes (Fig. 2A, 2B and 2C). Also, these two extracts showed a marked suppression of IL-6 and PGE2 release in human monocytes (Fig. 3A and 3B). On the other hand, the extracts containing thymoquinone had a remarkable effect enhancing the release of PGE2 from A549 human lung epithelial cells (Fig. 4).

Conclusion

Thymoquinone rich black seed extract (Nigellin® AMBER) showed the most potent and favourable effects in the context of asthma, due to their anti-inflammatory actions by suppressing the release of inflammatory mediators in human immune cells (T-lymphocyte and monocyte cells) as well as their possible bronchodilatory effect by enhancement of PGE2 release in A549 human lung epithelial cells.

References
  1. Koshak AE, Yousif NM, Fiebich BL, Koshak EA, Heinrich M. 2018. comparative immunomodulatory activity of Nigella sativa L. preparations on proinflammatory mediators: A focus on Asthma. Frontiers in Pharmacology, 9. doi: 10.3389/fphar.2018.01075